產(chǎn)品說(shuō)明 |
BD流式細(xì)胞技術(shù)在流式細(xì)胞儀器與流式抗體領(lǐng)域具有的技術(shù)和品牌影響力,對(duì)于擴(kuò)增后免疫細(xì)胞的質(zhì)量純度控制是非常關(guān)鍵的一一個(gè)環(huán)節(jié),運(yùn)用流式抗體鑒定免疫細(xì)胞表面標(biāo)記物的種類(lèi)較多,我們列舉幾種常用的流式抗體和同型對(duì)照供您參考。
技術(shù)參數(shù) |
產(chǎn)品品牌:BD
產(chǎn)品名稱(chēng):PE Mouse Anti-Human CD4 Clone RPA-T4 (RUO)
產(chǎn)品型號(hào):FITC/PE/APC/PerCP-CyTM5.5/APC-CyTM7/BV421/V450
產(chǎn)品貨號(hào):562843
產(chǎn)品規(guī)格:50 Tests
BD Mouse Anti-Human CD4 流式細(xì)胞儀/抗體
產(chǎn)品簡(jiǎn)介 |
RPA-T4克隆與CD4發(fā)生反應(yīng),CD4是一種59 kDa單鏈跨膜糖蛋白[人類(lèi)免疫缺陷病毒(HIV)的受體],存在于T輔助細(xì)胞/誘導(dǎo)細(xì)胞群上。該抗體與CD4抗原的D1結(jié)構(gòu)域(CDR1和CDR3表位)結(jié)合,與大約80%的胸腺細(xì)胞和45%的外周血淋巴細(xì)胞發(fā)生反應(yīng)。CD4也存在于外周血單核細(xì)胞的低密度。RPA-T4能夠阻斷HIV-1,gp120,并抑制合胞體的形成。
藻紅蛋白(PE)是紅藻中的一種輔助光合色素。它以240 kDa的蛋白質(zhì)形式存在于體外,每個(gè)分子含有23個(gè)藻紅蛋白發(fā)色團(tuán)。這使聚乙烯成為流式細(xì)胞術(shù)應(yīng)用中亮的熒光色素之一,但其光漂白特性使其不適合用于熒光顯微鏡。
BD Mouse Anti-Human CD4 流式細(xì)胞儀/抗體
準(zhǔn)備和儲(chǔ)存 |
在4°C下未稀釋儲(chǔ)存,并防止長(zhǎng)時(shí)間暴露在陽(yáng)光下。不要凍住。用親和層析法從組織培養(yǎng)上清或腹水中純化單克隆抗體。在jijia條件下將抗體與R-PE結(jié)合,去除未結(jié)合抗體和游離PE。
產(chǎn)品注意事項(xiàng):
此試劑已預(yù)先稀釋?zhuān)员阍诿看螠y(cè)試中按建議的體積使用。我們通常在100微升的實(shí)驗(yàn)樣品中使用1×10^6細(xì)胞(測(cè)試)。由于應(yīng)用不同,每位研究者應(yīng)滴定試劑以獲得jijia結(jié)果。
注意:dielvhuana在酸性條件下會(huì)產(chǎn)生劇毒的過(guò)氧化苯甲酸鈉。在丟棄之前,在自來(lái)水中稀釋疊氮化物化合物,以避免管道中積聚潛在的爆炸性沉積物。
訂貨信息 |
品牌 | 貨號(hào) | 產(chǎn)品名稱(chēng) | 規(guī)格 |
BD | 562843 | Horizon? BV605 Mouse Anti-Human CD4 | 50 Tests |
BD | 561840 | Pharmingen? APC Mouse Anti-Human CD4 | 25 Tests |
BD | 560769 | Horizon? V500 Mouse Anti-Human CD4 | 25 Tests |
BD | 550630 | Pharmingen? PE Mouse Anti-Human CD4 | 50 Tests |
BD | 565432 | Pharmingen? APC-Cy?7 Mouse Anti-Rat CD4 | 50 μg |
BD | 563737 | Horizon? BV650 Mouse Anti-Human CD4 | 50 Tests |
BD | 551980 | Pharmingen? APC Mouse Anti-Human CD4 | 50 Tests |
BD | 740962 | OptiBuild? BV786 Mouse Anti-Human CD4 | 50 μg |
BD | 565996 | Horizon? BB515 Mouse Anti-Human CD4 | 25 Tests |
BD | 565999 | Pharmingen? PE Mouse Anti-Human CD4 | 25 Tests |
BD | 566159 | Horizon? BV480 Mouse Anti-Human CD4 | 25 Tests |
BD | 742738 | OptiBuild? BUV395 Mouse Anti-Human CD4 | 50 μg |
BD | 566356 | Horizon? BV750 Mouse Anti-Human CD4 | 25 μg |
BD | 742652 | OptiBuild? BV605 Mouse Anti-Human CD4 v4 | 50 μg |
BD | 742654 | OptiBuild? BV711 Mouse Anti-Human CD4 v4 | 50 μg |
BD | 940001 | BD? AbSeq Oligo Mouse Anti-Human CD4 | 25 Tests |
BD | 566319 | Pharmingen? APC-Cy?7 Mouse Anti-Human CD4 | 25 Tests |
BD | 566911 | Pharmingen? FITC Mouse Anti-Human CD4 | 100Tests |
BD | 566004 | Pharmingen? PE-Cy?5 Mouse Anti-Human CD4 | 25 Tests |
BD | 566321 | Pharmingen? PerCP Mouse Anti-Human CD4 | 25 Tests |
BD | 566355 | Horizon? BV750 Mouse Anti-Human CD4 | 50 μg |
BD | 566924 | Pharmingen? PerCP Mouse Anti-Human CD4 | 100Tests |
BD | 566915 | Pharmingen? APC Mouse Anti-Human CD4 | 100Tests |
BD | 566907 | Horizon? BV421 Mouse Anti-Human CD4 | 100Tests |
BD | 560644 | Pharmingen? PE-Cy?7 Mouse Anti-Human CD4 | 50 Tests |